l , Immunoblot of indicated proteins in HeLa cells treated with erastin (5 M) and co-treated with either GSH (10 mM), GSHee (10 mM), Trolox (50 M) or Ferrostatin-1 (5 M) for 48 h
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For example, the deletion of tumor suppressor genes, such as PTEN and P53 or the activation of tumor genes, such as c-Myc and Ras, may induce changes in nutrient supply, metabolic enzymes, metabolic requirements and many other metabolic characteristics
Gene expression analysis by qPCR RNA was isolated from frozen tissues or fractionated adipocytes using TRIzol and the RNeasy Mini Kit (QIAGEN)
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